"Nonnative reptiles like Burmese pythons can be humanely killed on private lands at any time with landowner permission - no permit required- and the FWC encourages people to capture and humanely kill pythons from private lands whenever possible. There is no bag limit."
Many methods are available to kill nonnative reptiles. Regardless of the method you use, you must ensure the following two steps are completed to humanely kill a reptile:
Step 1: Your method should result in the animal losing consciousness immediately.
Step 2: You should then destroy the animal's brain by “pithing” which prevents the animal from regaining consciousness.